【摘要】 为了了解类c-kit原癌蛋白在多伊棺头蟋Loxoblemmus doenitzi Stein胚后精子发生中的表达、定位及可能的调控作用,采用常规免疫组织化学方法进行了相关研究。结果表明:处于减数分裂中期Ⅰ至末期Ⅱ的初级精母细胞的细胞膜上有类c-kit原癌蛋白阳性颗粒;精巢或受精囊内成熟精子头部也具有类c-kit原癌蛋白阳性颗粒。结果反映了类c-kit蛋白对于维持动物精子发生过程中减数分裂、精子成熟及受精能力具有特殊功能。更多还原

【Abstract】 The analogous proto-oncoproteins c-kit specially expressed in spermatocytes and sperm in postnatal spermatogenesis of Loxoblemmus doenitzi Stein were detected by immunohistochemistry.In meiosis,the analogous c-kit positive granules were on the cellular membrane of spermatocyte from metaphase Ⅰ to telophase Ⅱ.After meiosis,the analogous c-kit positive granules only existed in the head of the mature sperm in the testis and the spermatheca.The investigation indicated that the analogous proto-oncoprotein kit and its receptor as a signal transduction system may play some important role in keeping the meiosis process,the sperm maturation and the fertilization for L.doenitzi.These results are similar to those on mice which were marked by the proto-oncoproteins c-kit,but the difference still existed in some aspects.For example,there are many c-kit positive granules in mice’s spermatogonium and spermatoon during spermatogenesis,but there are not the analogous c-kit positive granules in L.doenitzi’s spermatogenous and spermatoon.It is so inferred that the functions of the analogous c-kit may be similar to the c-kit in regulating animal reproduction aspects.They may belong to the same gene’s product.更多还原