【摘要】 应用免疫组织化学和生物统计分析相结合的方法,对花胫绿纹蝗Aiolopus tamulus(Fabricius)和疣蝗Trilophidia annulata(Thunberg)配子发生过程中原癌基因c-kit特异表达进行了比较研究。结果表明:(1)精子发生过程中,精原细胞、初级精母细胞、次级精母细胞和成熟精子中均有不同程度的c-kit蛋白表达,精巢末端还有较粗大的阳性颗粒分布;(2)卵子发生过程中,第1~6阶段卵母细胞中有不同程度的c-kit蛋白特异性表达,但随着卵黄发生的开始逐渐消失;(3)2种蝗虫配子发生过程中c-kit表达存在种间差异。更多还原

【Abstract】 The special expression of c-kit protein in gametogenesis of Aiolopus tamulus(Fabricius)and Trilophidia annulata(Thunberg)collected from Siping,Jilin,Northeast China was studied by using immunohistochemistry and biostatistics.The results showed that:(1)In spermatogenesis,there were different expression levels of c-kit protein in spermatogonia,primary spermatocytes,second spermatocytes and mature sperms,and the biggest positive granules were found at the end of spermary;(2)In oogenesis,there were different expression levels of c-kit protein from the 1st to 6th stage of oocytes,and the positive granules gradually disappeared in vitellogenesis;(3)The expression of c-kit protein in gametogenesis was significantly different between the two species.更多还原