【摘要】 利用22对AFLP引物组合对我国9个省市的11个东方蜜蜂种群和1个西方蜜蜂种群的39个个体基因组DNA的遗传变异进行了研究;根据AFLP分析结果,采用GeneScan3.0软件、群体遗传数据分析包(Hickoryv1.0.4)和群体遗传变异分析程序(AFLP-SURV1.0),分别计算了39个个体间的遗传相似系数和各蜜蜂种群的Nei’s遗传距离、Reynolds遗传距离和成对的固定指数Fst,并构建了各自的UPGMA聚类关系图。结果表明:AFLP标记具有很高的多态检测效率,适合于蜜蜂种群遗传多样性分析和品种鉴定。蜜蜂种间的遗传分化明显,亲缘关系较远。中国境内不同地理型东方蜜蜂群体间存在着广泛的遗传变异。UPMGA聚类关系图显示,海南东方蜜蜂由于长期的海岛隔离,已经形成了一个独特的类群,支持了通过形态学认定的海南东方蜜蜂为东方蜜蜂的一个新亚种。更多还原

【Abstract】 The genetic variation and differentiation among 11 populations of Apis cerana Fabricius and one population of Apis mellifera ligustica Spinola from 9 provinces (cities) of China were analysed by a total of 22 AFLP primer combinations. The genetic similarity coefficients among 39 individuals, and Nei’s distance, Reynolds distance, pairwise F_ st among the populations were calculated from AFLP data. Dendrogram trees among individuals and/or populations were constructed based on UPGMA clustering analysis. The results indicated that AFLP analysis had high assaying efficiency and provided a valuable tool for studying genetic diversity and breed characterization in honeybees. Remarkable genetic differentiation, indicated by the genetic similarity coefficients, was found between A. cerana and A. mellifera. Extensive genetic variation was also existed among the nine populations of A. cerana, indicating that A. cerana was rich in genetic diversity. The UPGMA tree suggested that the honeybees of Hainan province had evolved into a unique subspecies because of island isolation, which supported the results based on morphological research.更多还原