【摘要】 目的明确3种中药有效成分三七皂甙、黄芪皂甙及黄芩甙单用或配伍对经过幽门螺杆菌培养滤液转化后的永生化人胃黏膜上皮GES-1细胞(简称HP细胞)的凋亡诱导作用。方法以三七皂甙、黄芪皂甙及黄芩甙单独或联合作用HP细胞,AnnexinV及PI双染,流式细胞仪检测药物对HP细胞凋亡/坏死的影响;电镜和Hoechst33258及PI双染荧光显微镜观察凋亡细胞形态。结果三七皂甙、黄芪皂甙及黄芩甙单用可引起HP细胞凋亡及死亡比例增加并呈一定的时间依赖性,配伍应用作用强于单独应用(与对照组比较P<0.05),增强程度从大至小依次为三药合用>三七皂甙黄芪皂甙合用>黄芪皂甙黄芩甙合用>三七皂甙黄芩甙合用。结论3种中药有效成分单用及配伍对HP细胞有明显的毒性作用;引起细胞死亡的机制部分是通过诱导凋亡实现的;不同药物配伍应用的增效程度不同。更多还原

【Abstract】 Objective To investigate the effects and the synergistic effects of Panax Notogingseng Saponin (PNS), Astragalin Saponin (AS) and Baicalin (Ba), the main active components from 3 Chinese herbal medicines, on the apoptosis of a Helicobacter pylori supernatant transformed human gastric epithelial cell line (HP cells).Methods An immortalized human gastric epithelial cell line, GES-1 cells, was transformed by Helicobacter pylori supernatant twice, and the transformed cells were named as HP cells. HP cells were treated with the above 3 medicines alone or with different combinations of them. Cell apoptosis and/or necrosis were detected by electron microscopy, fluoroscopy and flowcytometry(Annexin V and Propidine iodide labeled).Results PNS, AS and Ba could induce apoptosis and/or necrosis of HP cells in a time-dependence manner. The effects were greatly enhanced when two or three of these medicines were used together (P<0.05 as compared with negative control cells). The order of enhancive degree from high to low was PNS+AS+Ba, PNS+AS, AS+Ba and PNS+Ba.Conclusion PNS, AS, Ba and their combinations have obviously cytotoxic effect on HP cells. This effect is partially caused by inducing apoptosis. Different combinations between or among these medicines induce cell death in different degrees.更多还原