【摘要】 目的建立血浆中清脑宣窍方有效部位中栀子苷、人参皂苷Rg1、Rb1及三七皂苷R1的HPLC血药浓度测定方法。方法血浆样品用C18固相柱萃取,以亥茅酚苷为内标,选用Agilent Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm),流动相为乙腈-水二元梯度洗脱,柱温为30℃;流速为1.0mL/min;检测波长为203nm。结果各成分的最低检测浓度(S/N>3)在0.2~0.9mg/L之间,平均回收率均在90%以上,日内、日间精密度及稳定性的RSD均小于10%。结论该方法简便、准确,可作为清脑宣窍方血药浓度定量分析方法。更多还原

【Abstract】 Objective To establish a method for determinating the concentration of geniposide, ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in the effective fraction of Qingnaoxuanqiao Formula in beagle dog plasma by HPLC. Method Plasma samples were extracted by C18 solid phase column. Sec-o-glucosylhamaudol was taken as internal standard. Agilent Eclipse XDB-C18 chromatography column (4.6 mm×250 mm, 5 μm) was applied. The gradient mobile phase was HCN-H2O system with column temperature 30 ℃ and flow rate 1.0 mL/min. The detecting wavelength was 203 nm. Results The limited concentrations of each component were between 0.2～0.9 mg/L; the average recoveries were all above 90%; RSDs of intra-day and inter-days accuracies and stability were all below 10%.Conclusion The method is simple and accurate, and can be used as the quantitative analysis method for the 4 components in the effective fraction of Qingnaoxuanqiao Formula in plasma .更多还原