【摘要】 基于PCR微流控芯片,研究和设计了一套多通道温度控制系统,实现了温控功能并应用于PCR扩增试验。系统由硬件电路搭建和控制软件编写共同实现。温度信号由AD590采集,经过CD4051选通,由TLC0832将模拟信号转换为数字信号,传送给AT89C2051。单片机根据负反馈算法对信号进行处理,得到控制指令,经过TLC5620得到模拟信号,控制驱动电路。利用汇编语言编写功能软件,包括A/D,D/A转换程序、跳转程序和系统闭环程序。通过自主设计的硬件电路和控制软件,实现了微型PCR微流控芯片的多通道温度控制系统。实验结果表明,系统符合PCR扩增原理的要求,升温迅速,控温准确,可用于PCR扩增试验。更多还原

【Abstract】 A temperature-controlled system with multichannel of PCR biochips is designed for achieving the function of temperature control and applying in the PCR experiment.The hardware and the software actualize the system function.Temperature signals are collected by AD590,and then are selected by CD4051.The analog signal is transferred to the digital signal by TLC0832 and transmitted to AT89C2051.Microcontroller treats the signals and implements temperature control,according to negative feedback algorithm.TLC5620 is used to change the control command to analog signal,and then controlle the drive part.The assembly language is used to write the A/D conversion,D/A conversion and closed loop program.The experimental results show that the system meets the requirements of PCR principle.It is accurate and the rise/fall rates of temperature are rapid.更多还原