【摘要】 目的了解本院血培养分离到的病原菌菌种及对抗生素的耐药性。方法利用Bact/Alert3D全自动血培养仪及其配套的血培养瓶对636份标本进行血培养,阳性血培养转种后用MicroScan A/s-4细菌鉴定及药敏测试仪进行测试。结果636份血培养标本中,检出病原菌65株(10.22%),革兰阳性球菌占40%,革兰阴性杆菌占55.4%,真菌占4.6%,葡萄球菌属和肠杆菌科的细菌是本院菌血症和(或)败血症的主要病原菌。革兰阳性球菌中未发现对万古霉素耐药的菌株,革兰阴性杆菌对亚胺培南有高度敏感性,但对常用抗生素有较普遍的耐药性。结论血培养检出菌种复杂,耐药率高,应加强血培养中致病菌及其对抗生素的耐药性监测。更多还原

【Abstract】 Objective To explore the isolated bacteria and their drug resistance from blood culture samples in our hospital.Methods 636 blood samples were cultivated by Bact/Alert3D Automated Blood Culture System and appropriative culture bottles.The positive blood cultures were reinoculated. Then species identification and drug sensitivity tests were performed by the MicroScan A/s-4.Results Among the 636 blood samples, 65 strains of phthogens were isolated (10.22%).40% were gram-positive cocci, 55.4% were gram-negative bacilli, and 4.6% were fungi.Staphylococcus and enterobactericaeae were main pathogens in bacteremia and (or) septicaemia in our hospital . No strains resistant to vancomycin was found in gram-positive cocci. Gram-negative bacilli were highly sensitive to imipenem.But they were resistant commonly to antibiotics used often.Conclusion The kinds of isolated bacteria were complex from the positive blood cultures and their drug-resistance rates were high. More attention should be paid to the detection and surveillance of bacterial resistance in blood culture.更多还原