【摘要】 目的:探讨慢性肾小球肾炎(CGN)患者红细胞补体受体Ⅰ型分子(CR1)数量表达和黏附活性及其基因多态性的变化及临床意义。方法:采用聚合酶链反应(PCR)和HindⅢ酶切技术测定红细胞CR1分子基因型,采用酶联法定量测定红细胞CR1分子的数量,以红细胞天然免疫黏附试验测定红细胞CR1分子黏附活性。结果:50例CGN患者红细胞CR1基因多态性分布(HH:56.0%,HL:32.0%,LL:12.0%)与50名健康人(HH:68.0%,HL:26.0%,LL:6.0%)比较无统计学差异;CGN患者红细胞CR1数量表达和黏附活性明显低于健康人(P<0.01)。并且CGN患者红细胞CR1高、中、低表达基因型的数量表达和黏附活性均低于健康人高、中、低表达基因型(P<0.05)。结论:CGN患者红细胞CR1数量表达和黏附活性的变化主要是后天因素引起,红细胞CR1数量表达和黏附活性与CGN的发生和发展有着非常密切的关系。更多还原

【Abstract】 Objective:To study the changes of quantitative expression,adhering activity and genomic density polymorphism of complement type Ⅰ in erythrocytes(CR1)of patients with chronic glomerulonephritis(CGN)and clinical significance.Methods:Polymerase chain reaction(PCR),Hind Ⅲ restriction enzyme digestion,the quantitative assay of ECR1 and the adhering activity assay of CR1 in erythrocytes were used.Results:No difference was observed between the patients with CGN(%)and healthy individuals(%)in the spot mutation rate of CR1 density gene.The number and the adhering activity of CR1 in patients with CGN(and)were significantly lower than those in healthy individuals(and).The number and the adhering activity of ECR1 in high expression genomic type(HH)CGN were obviously lower than those of healthy individuals.Conclusion:Change of erythrocytes CR1 quantitative expression,adhesive active in patients of CGN is mostly acquired through central peripheral mechanisms.The change of erythrocytes CR1 quantiative expression and adhesive active are closely related to the patients wity CGN.更多还原