【摘要】 对降低皂质芦荟诱导愈伤组织褐化情况的研究表明,外植体接种前进行预处理对控制诱导愈伤组织的褐化有一定的帮助。其中,以在PVP(2 g/L)溶液中浸泡15 m in的效果最为显著;暗培养能显著减轻褐化的发生,有利于愈伤组织的生长;培养基中附加抗氧化剂和吸附剂对愈伤组织的褐化有不同的作用效果,抗氧化剂要优于吸附剂。以收获愈伤鲜重为基础,考察其抗褐化能力为:L-谷氨酰胺(5 mg/L)>AgNO3(15 mg/L)>Vc(2 mg/L)>PVP(0.5 mg/L)>柠檬酸(5 mg/L)>Na2S2O3(10 mg/L)>空白>活性炭(10 mg/L)。更多还原

【Abstract】 The study on the reducing browning of callus induced from Aloe saponaria Haw indicated that: pretreatment before inoculation was useful for reducing callus browning.Soaking explants in pvp(2 g/L)15 min showed distinct effect;darkness culture reduced browning greatly,and benefited callus’s growth;media with addition of antioxidant or sorbent showed different effects,antioxidant was better than sorbent.On the basis of fresh callus weight acquiring,the browning restraining ability was as follows: L-Glutamine(5 mg/L)>AgNO3(15 mg/L)> Vc(2 mg/L) >PVP(0.5 mg/L) > Citric acid(5 mg/L) > Na2S2O3(10 mg/L) > blank > Ac(10 mg/L).更多还原