【摘要】 目的:观察重组腺病毒介导的血管内皮抑素基因在体外转染A549细胞后的表达。方法:以复制缺陷型重组腺病毒为载体,将血管内皮抑素基因导入肺癌A549细胞,以免疫组织化学法、Western印迹法和ELISA法检测内皮抑素在A549细胞中的表达。结果:免疫组化染色、ELISA法和Western印迹法检测:A549细胞中内皮抑素蛋白表达阳性,Ad.Null组及PBS组内皮抑素蛋白未见有表达。结论:Ad.Endostatin转染A549细胞可表达具有生物学活性的内皮抑素。更多还原

【Abstract】 Objective:To observe the expression of endostatin gene mediated by recombinant adenovirus. Methods:Endostatin gene was transfected into lung carcinoma cells in vitro mediated by recombinant adenovirus. The expression products of the gene were detected by immunohistochemistry assay, Western-Blot and ELISA. Results:The endostatin could be found in lung carcinoma cells from immunohistochemistry assay, Western-Blot and ELISA, while nome was found in the other group. Conclusion:The recombinant adenovirus can effectively express biologically active endostatin.更多还原