【摘要】 目的:探讨β-1,4-半乳糖基转移酶I与雪旺细胞衰老的相关性。方法:以不同浓度的β-1,4-GalT-I表达质粒转染雪旺细胞,观察衰老相关半乳糖苷酶染色情况。以Ha-Ras转染雪旺细胞诱导衰老,采用Northern blot方法检测转染后不同时间β-1,4-GalT-I表达变化。结果:随着转染β-1,4-GalT-I表达质粒浓度的提高,细胞衰老相关半乳糖苷酶染色阳性率增高;随着Ha-Ras转染时间的延长,β-1,4-GalT-I表达逐渐增高。结论:β-1,4-GalT-I与雪旺细胞的衰老相关,并可能在周围神经雪旺细胞的增殖调节中发挥重要作用。更多还原

【Abstract】 Objective:To study the relations between β-1,4-GalT-I and the aging of Schwann cells.Methods:The stain of senescence-association β-galactosidase was studied in Schwann cells transfected with β-1,4-GalT-I expression plasmids of various concentrations.Northern blot was used to investigate the changes of β-1,4-GalT-I in Schwann cells transfected with the Ha-Ras expression plasmids of various time.Results:The stain of senescence-association β-galactosidase increased when the concentration of β-1,4-GalT-I expression plasmids increased,and the expression of β-1,4-GalT-I mRNA increased with the increased days of transfected Ha-Ras in Schwann cells.Conclusion:It is suggested that some relations exist between β-1,4-GalT-I and the aging of Schwann cells,and that may play an important role in regulating the proliferation of Schwann cells in peripheral nerves.更多还原