【摘要】 取孵化15 d鸡胚及出壳7 d内和7 d后雏鸡的睾丸,分别采用二酶二步消化法(胶原蛋白酶+胰蛋白酶)和三酶二步消化法(胶原蛋白酶+透明质酸酶/胰蛋白酶)分离睾丸细胞。结果表明:同一时期睾丸细胞的分离效果,二酶二步消化法与三酶二步消化法差异显著;不同时期采用二酶二步消化法分离睾丸细胞,孵化15 d的分离效果显著优于出壳7 d内,极显著优于出壳7 d后,出壳7 d内极显著优于出壳7 d后。Percoll离心法提纯结果表明:密度梯度为27%~35%的条带中睾丸细胞纯度较高。在体外无饲养层的条件下分别培养经提纯与未经提纯的睾丸细胞,结果表明未经提纯的细胞具有较高的存活力。更多还原

【Abstract】 The separation methods for both of collagenase type1+hyaluronidase/TE and collagenase type1+TE+TE with different stage of embryo development were studied.The results showed that: there was significant difference between the method 1 and method 2 at the same stage;The amount of testicle cells purified from different stage showed significant or very significant difference with the method 2.The result of Percoll centrifugal was that in the 27%-35% density cincture,the numbers of testis cells were highest.Culturing the cells without feeder layer in vitro showed that the viability of unpurified testis cells was better than purified testicle cells.更多还原