【摘要】 以16S rRNA、mapA和ceuE为靶基因,建立检测空肠弯曲菌和结肠弯曲菌的多重PCR方法。特异性试验能分别扩增出空肠弯曲菌和结肠弯曲菌的特异性条带,其他参考菌株均未扩增出条带;敏感性试验表明,基于增菌培养和选择性培养的多重PCR方法对弯曲菌检测限为10 mL或10 g模拟污染样品,最低可检测0.925 pg.μL-1空肠弯曲菌DNA和1.050 pg.μL-1结肠弯曲菌DNA。以国家标准为参照,应用该方法对180份生鲜鸡肉样品进行检测,结果表明多重PCR方法具有快速、简便、特异性强和灵敏度高等特点,可克服传统生化鉴定和血清学方法的不足,为弯曲菌检测提供新的技术。更多还原

【Abstract】 A multiplex PCR(m-PCR) targeting 16S rRNA gene,mapA gene and ceuE gene for the detection of Campylobacter jejuni and Campylobacter coli was developed.Species-specific product could be detected after amplification of the DNA template of C.jejuni and C.coli,while other bacteria strains could not be detected.The m-PCR assay could detect as low as 10 mL or 10 g,artificial contaminated samples and its detect limit of C.jejuni and C.coli were 0.925 and 1.050 pg·μL-1 DNA template respectively.180 of fresh chicken samples were detected for the C.jejuni and C.coli by the m-PCR,and the result was coincided with that of the national standard method for Campylobacter.It was suggested that the m-PCR was highly specific and sensitive,and could overcome the shortcomings of biochemical and serological identification.更多还原