【摘要】 用傅立叶变换近红外技术非破坏性测定油菜籽的油酸、芥酸和含油量,并建立数学模型。内部交叉验证表明:该方法测定上述3个品质参数的定标方程决定系数分别为0.979 2、0.992 4和0.974 9,均方差依次为1.96、1.56和0.67,与化学测定方法有类似的准确性和精确性。外部检验结果表明,油酸、芥酸和含油量的近红外测定值与化学测定值之间相关系数分别为0.99,0.99和0.97,平均绝对误差和相对误差分别为2.31%、0.29%、0.76%和4.21%、3.40%、1.90%。装样质量对近红外测定值精度影响试验结果表明:当装样质量为0.50 g时,对测定值影响较大;当装样质量为1.00~1.50 g时,对测定结果影响较小;当装样质量大于1.50 g时,近红外测定值与化学测定值基本一致。重复性分析结果表明,近红外光谱分析法重复性好,达到国标要求。近红外光谱分析技术完全可以用来快速、简便、非破坏性测定育种材料,是油菜低世代材料品质筛选的重要手段。更多还原

【Abstract】 The method for analysis of oleic acid,erucic acid and oil content of rapeseed without being damaged by using near-infrared reflectance spectroscopy(NIRS)was evaluated.Both accuracy and prediction of NIRS method were similar to those of the standard chemical method.Good repeatability of NIRS was also seen in the experiment.The determination coefficients(R2)and RMSECV of oleic acid,erucic acid and oil content were 0.979 2,0.992 4,0.974 9 and 1.96,1.56,0.67 respectively.Correlation coefficients of them in rapeseed were 0.99,0.99,0.97 between NIRS and standard chemical methods,respectively.Mean absolute error and relative error were 2.31%,0.29%,0.76% and 4.21%,3.40%,1.90%.There was a relationship between sample weight and precision by NIRS.When sample weight was less than 0.50 g in ampere bottle,the result was not as good as that determined by sandard method.When sample weight was 1.00—1.50 g,the result was acceptable.When sample weight was more than 1.50 g,the result was the best.NIRS can be used as a simple and quick mothod for selection of rapeseed germplasm without damaging the materials.更多还原