【摘要】 构建了乙肝病毒表面抗原基因(HBsAg)植物表达载体,通过冻融法将HBsAg基因转入到发根农杆菌LBA1314中,采用叶盘法将HBsAg基因导入到烟草中,获得了转基因烟草发根.对转基因烟草发根的GUS检测结果表明:转HBsAg基因烟草发根可以染成蓝色,而非转基因烟草的根没有染色反应.这说明gus基因在转HBsAg基因烟草发根获得了表达.更多还原

【Abstract】 A plant expression carrier containing HBsAg(hepatitis B virus surface antigen)gene was constructed in a plasmid,the plasmid was mobilized into a rhizogenes Strain LBA 1314 by freeze-thaw method,the HBsAg gene was planted into tobacco by leaf disc via Agrobacterium tumefaciens LBA 4404 as a vehicle and the tobacco hairy roots were received.Histochemical staining of GUS activity was made in the transgenic tobacco hairy roots:The roots with HBsAg genes can be dyed into bule,while those without have no reflection of color.So the conclusion can acquire that the gene has its expression in the roots.更多还原