【摘要】 目的探讨前列腺癌中血小板源内皮细胞生长因子(PD-ECGF)的表达与新生血管形成的关系。方法用免疫组化技术和Western blot方法检测22例前列腺癌组织中PD-ECGF蛋白的表达和微血管计数(MVD)。结果前列腺癌中PD-ECGF阳性表达率为54.5%(12/22),表达位于肿瘤组织的基质细胞中,肿瘤细胞中均未见表达。在22例前列腺癌组织中平均MVD为71.21±31.14/100倍视野,PD-ECGF的表达与MVD间存在显著地相关性(r=0.50,P=0.005)。结论PD-ECGF在前列腺癌组织中呈高表达与前列腺癌的血管生成显著相关,可促进前列腺癌组织中新生血管的形成。更多还原

【Abstract】 Objective To observe the expression of platelet-derived endothelial cell growth factor(PD-ECGF) and its relation to angiogenesis of prostate cancer.Methods The expression of PD-ECGF and microvessel density(MVD) were studied in 22 prostate cancer patients by using immunohistochemical and Western blot technology.Results Positive expression of PD-ECGF protein detected in 12(54.5%) of the 22 cases.The positive staining for PD-ECGF was seen in the stromal cells within cancer tissue.However,cancer cells were negative for PD-ECGF in all cases.The mean MVD in 22 prostate cancer cases was 71.21±31.14 vessels per 100 high field of vision.Tumors with a high expression of PD-ECGF showed a high MVD and the correlation between PD-ECGF immunopositivity and microvessel density was highly significant(r=0.50 P=0.005).Conclusion The PD-ECGF is strongly expressed in most prostate cancer and was associated with neovascularization.PD-ECGF is likely to contribute to the extensive neovascularization.更多还原