【摘要】 激光制备微流控芯片工艺研究的生物聚合酶链式反应(PCR)荧光分析方法是利用生物PCR反应中荧光的变化规律特性,从检测结果与参照检测标准的比较依据出发,在生物芯片的实际使用工作状态下获得分析数据。对造成达不到设计工作状况现象的内在混合诸方面工作参数进行分解分类和分析。用于微流控芯片工艺的生物PCR荧光分析方法的相关装置采用了多光路光纤校准,获得了荧光检测良好的重复性和稳定性。该装置对标准模板上模拟微通道中不同荧光信号的分辨率(平台区荧光强度信号值>10×本底信号区荧光强度信号值)表明:已建立的生物PCR荧光分析装置能分析微流控芯片的工艺参数,实现了用于激光制备生物微流控芯片工艺的PCR荧光分析方法。更多还原

【Abstract】 The biological polymerase chain reaction(PCR) fluorescence analysis methods for technics in manufacturing micro-continuous-flow chip by laser is described.This method,using the characteristic of changed rule of fluorescence in biological PCR reaction,based on the comparison between the detection result and the reference detection standard,obtains the analysis data under the working state in actual use of biological chip,disassembling,classifying and analyzing the intrinsic mix various aspects operational parameter in the phenomenon not achieving the design working condition.The correlative device of biological PCR fluorescence analysis methods for the technics manufacturing the micro-continuous-flow chip,using the multi-paths of rays optical fiber calibration,obtains the fluorescence detection accuracy of repetition,stabilization and so on.The resolving power(most fluorescence signal > 10 × least fluorescence signal) indicates in this device of different fluorescence signals in simulation micro channels on standard templates that it has established correlative device of biological PCR fluorescence analysis methods,has achieved the concrete technics parameter level of analyzing and manufacturing the micro-continuous-flow chip,and realized the start of the biological PCR fluorescence analysis methods in technics of the micro-continuous-flow chip by laser.更多还原