【摘要】 以7份高粱自交系幼胚为外植体,研究了不同基因型对组织培养的反应和植株再生的差异。结果表明:不同高粱基因型间愈伤组织诱导率没有明显差异,但胚性愈伤组织百分率、不定芽分化率以及再生频率有明显差异。其中YN336A、YN267R、YN510R和YN338A的再生效果较好,其不定芽发生率、不定根发生率及移栽成活率分别为64.4%-72.8%,76.2%-100.0%和36.3%-80.0%。对只生根不生芽的基因型,加入1-2 mg/L KT有明显抑制生根、促进生芽的效应。更多还原

【Abstract】 Explants of immature embryos of seven genotypes of sorghum inbred lines were examined for their response in tissue culture and plant regeneration.The results indicated that there were no significant differences of induction frequency among different genotypes,but the frequency of regenerable callus formation,shoot regeneration and plant regeneration was significantly different.YN336A,YN267R,YN510R and YN338A showed good performances under regeneration culture conditions,frequency of shoot formation,root formation and transplant survive was in the range of 64.4%～72.8%,76.2%～100.0% and 36.3%～80.0%,respectively.For the genotypes that rooted rather than shot,1～2 mg/L KT could effectively inhibit rooting and promote shooting.更多还原