【摘要】 将苏云金芽孢杆菌伴孢晶体蛋白的基因(Bacillus thuringiesis,简称Bt)通过甘氨酸接头(Gly4Ser)3与一种人工合成的抗菌肽(antimicrobial peptides,AMP)基因与相融合,编码一种新的杀虫,并具有抗菌的蛋白。把融合基因(NAMP-Bt)连接到原核表达载体pET-28a和植物表达载体pBI-121上,经过限制性酶切分析和PCR鉴定,结果表明含有融合基因的原核和真核重组表达质粒均已构建成功,并将该融合基因转入烟草,已获得抗性小植株。更多还原

【Abstract】 Antifungal protein gene and Bacillus thuringiesis gene were ligated by the linker of（Gly4Ser）₃,which encode a new anti-insect and antifungal protein.The fusion gene（NAMP-Bt） were ligated into prokaryotic expression vector and plant expression vector.These recombinant plasmids pET-NAMP-Bt and pBI-NAMP-Bt were confirmed by restriction enzyme and PCR analysis and the results showed that these recombinant plasmids were constructed successfully.The fusion gene was induced into tobacco,we had obtained transgenic tobacco.更多还原