【摘要】 目的检测RCAS1在正常宫颈、宫颈上皮内瘤样病变及宫颈癌组织中的表达,探讨RCAS1在宫颈癌发生发展中的作用。方法采用免疫组织化学S-P法,分别检测89例宫颈癌、82例子宫颈上皮内瘤样病变(CIN)及24例正常宫颈组织中RCAS1蛋白的表达。临床分期:Ⅰ期34例,Ⅱ期41例,Ⅲ～Ⅳ期14例;病理分级:G137例,G228例,G324例;病理类型:鳞癌58例,腺癌31例;有盆腔淋巴结转移者36例;肿瘤直径:≥4cm31例,<4cm58例。另取同期因子宫肌瘤行子宫全切术的正常宫颈组织标本24例作为对照。结果免疫组化结果显示:正常宫颈组织、CINⅠ级、CINⅡ～Ⅲ级和宫颈癌组织中RCAS1蛋白的阳性表达率分别为0、31.4%、44.7%和77.5%。宫颈癌组与正常宫颈组和CIN各组间比较差异有显著性(P<0.05);正常宫颈组与CIN组比较差异有显著性(P<0.05)。RCAS1阳性表达率在低分化宫颈癌组显著高于高、中分化癌组(P<0.01);RCAS1阳性表达率在淋巴结转移组中为94.4%,而在无淋巴结转移组中为66.0%,两者相比差异有显著性(P<0.01);肿瘤直径≥4cm的宫颈癌组织中RCAS1水平明显高于肿瘤直径<4cm者(P<0.05);但RCAS1阳性表达与患者年龄、临床分期及组织学分型无关(P>0.05)。结论RCAS1表达与宫颈癌的发生、发展密切相关。随着宫颈病变恶性程度的进展RCAS1的表达逐渐增强,RCAS1的过度表达可作为宫颈癌恶性程度和不良预后的重要指标。更多还原

【Abstract】 Objective To investigate the expression of RCAS1 in the tissues of normal cervix,cervical intraepithelial neoplasia(CIN)and cervical cancer,and to explore their role in the pathogenesis of cervical carcinoma.Methods The expression of RCAS1 protein was analyzed by immunohistochemical S-P method in 89 cases of cervical carcinoma,82 cases of CIN,and 24 cases of normal cervical specimens.Results Immunohistochemistry showed that the expression rate of RCAS1 in normal cervix,CINⅠ,CINⅡ-Ⅲ and cervical cancer were 0.00,0.314,0.447 and 0.775,respectively.RCAS1 expression tended to increase along with tumor progression.The expression of RCAS1 in cervical cancer was significantly related to the histological grade,the tumor in different sizes(≥4cm or <4cm)and metastasis for lymph nodes,but the expression of RCAS1 showed no significant difference(P>0.05)betwan patients’ age,clinical stage and histological classification.Conclusions The data suggested that RCAS1 might play an important role in the pathoqenesis and development of cervical carcinoma.Over expressed RCAS1 could be regard as an important parameter in malignant degree and poor prognosis of cervical cancer.更多还原