【摘要】 目的观察一氧化氮(NO)和细胞间黏附分子-1(ICAM-1)在高脂血症及动脉粥样硬化(AS)动物模型中的表达,探讨高脂血症及AS与血管内皮功能和ICAM-1的关系。方法采用高脂饮食饲养家兔建立高脂血症和AS动物模型,正常饮食组为对照组;两组动物于0、8和16周分别取静脉血,检测血清中胆固醇(TC)、低密度脂蛋白(LDL)、NO和ICAM-1水平,并观察TC和LDL与NO和ICAM-1的相关性;16周后处死动物,免疫组化法和RT-PCR检测ICAM-1在主动脉粥样硬化斑块中的表达。结果饲养8周时,高脂饮食组血清TC和LDL明显增加(P<0.01),NO和ICAM-1无明显变化;饲养16周时,NO水平明显下降(P<0.01),ICAM-1水平明显增高(P<0.01)。TC和LDL与NO呈负相关,与ICAM-1呈正相关。结论高脂血症及AS造成血管内皮功能失调,导致NO和ICAM-1的异常表达;NO和ICAM-1参与了AS的形成。更多还原

【Abstract】 Objective To study the expression of nitricoxide(NO) and intercellular adhesion molecule-1(ICAM-1) in the animals with hypercholesterolemia and atherosclerosis(AS) in order to investigate the relationship of hypercholesterolemia with AS and vascular endothelium function and ICAM-1. Methods Fed rabbits with high-cholesterol diet to establish the models of hypercholesterolemia and AS,and the control group was fed with normal diet;Blood was collected from vein in 0,8 and 16 weeks in two groups,and the concentrations of TC,LDL,NO and ICAM-1 were measured;The correlation among TC,LDL and NO,ICAM-1 was observed;After 16 weeks,the rabbits were put to death,and immunohistochemistry analysis and RT-PCR were performed to measure the expression of ICAM-1 in the aortas.Results TC and LDL in 8 weeks were higher in the high-cholestrol diet group,but NO and ICAM-1 had no change;NO decreased and ICAM-1 increased in 16 weeks(P<0.01);TC and LDL were negatively correlated with NO and positively correlated with ICAM-1;The expression of ICAM-1 in the high-cholesterol diet group was higher than that of control group(P<0.01).Conclusion Hypercholesterolemia and AS lead to vascular endothelium disorder and abnormal expression of NO and ICAM1;NO and ICAM-1 may contribute to the formation of AS.更多还原