【摘要】 在高精料日粮日粮条件下(6:4),利用体外静态模拟发酵法,设计4个水平3个重复,通过分别添加0 mg/L、50mg/L、100mg/L、150mg/L海南霉素于发酵瓶中,研究海南霉素对反刍动物瘤胃发酵调控的影响。结果表明经恒温培养箱39℃培养72h,在设定的时间点测定pH值、产气量、氨氮含量、挥发性脂肪酸、中性洗涤纤维,发现在体外条件下海南霉素各处理组在0~24h内能减缓pH的下降,和对照组比差异都显著,但24h后却影响不大;产气方面,各时间点的海南霉素各处理组的产气量都极显著低于对照组,在72h时间点上,50mg/L、100mg/L、150mg/L海南霉素组分别比对照组减少产气25.1%、28.2%、20.9%,和对照组都差异极显著(P<0.01);海南霉素能减少瘤胃液中氨氮含量,72h后,50mg/L、100mg/L与150mg/L处理组比对照组氨氮浓度分别减少31.9%、33.4%、28.7%,差异极显著(P<0.01);同时可以改善瘤胃发酵类型,海南霉素各处理组能降低乙酸丙酸比例,差异显著(P<0.05),而72h后各处理组能提高总挥发性脂肪酸的生成量,但影响不大(P>0.05);对中性洗涤纤维NDF影响不大。更多还原

【Abstract】 In this experiment,the effects of Hainanmycin on artifical rumen fermentation in the high concentrate diet was studied in vitro.4 defferrent feeding levels of Hainanmycin with 3 repetitions were designed,which is 0 mg/L,50mg/L,100mg/L and 150mg/L.During 72h foster at 39℃,the results showed that the pH values in the fermentation liquids of the four level were no significant difference(P>0.05) after 72h fermentation,but between 0~24h Hainanmycin levels could slow down the drop of PH;Hainanmycin levels could reduce volume of gas production,the 50mg/L、100mg/L and 150mg/Llevels could reduce 25.1%、28.2%、20.9% each(P<0.01).50mg/L、100mg/L and 150mg/L defferrent levels of Hainanmycin could also reduce NH3-N concertation 31.9%、33.4%、28.7% comparing control(P<0.01);And the radio of acetate to propionate(A/P) was decreased significantly by using Hainanmycin(P<0.05),and at 72h,Hainanmycin each level could increase the amount of total VFA a little(P>0.05);But Hainanmycin did no much influence on NDF.更多还原