【摘要】 目的了解中国单核细胞增生李斯特菌(Lm)的毒力基因分布情况。方法对Lm的6个毒力基因(hly、prfA、plcB、inlA、actA、iap)分别设计聚合酶链反应(PCR)引物,建立PCR检测方法。对hly、plcB、iap3个基因的PCR方法进行了特异性试验。结果对国内多个地区多种来源的118株Lm进行了PCR检测,结果表明除2株菌不含prfA基因外,其他菌株均包含6个毒力基因。PCR引物特异性试验结果表明hly、plcB、iap3个基因的PCR引物特异性良好,可用于Lm的鉴定。结论Lm普遍具有毒力基因,具有潜在的爆发风险,应加强对其监测。更多还原

【Abstract】 Objective The study was conducted to investigate the distribution of virulence genes of Listeria monocytogenes in China. Methods Six pairs of PCR primers were designed for the six virulence genes(hly、prfA、plcB、inlA、actA、iap) of L. monocytogenes in the PCR test. Specific tests for the PCR of gene hly, plcB, iap were conducted. Results PCR tests conducted in 118 strains of L. monocytogenes indicated that all strains contained the six virulence genes except that two of them lacked prfA gene. Specific tests indicated that the 3 pairs of primers for hly, plcB, iap had good specificity and could be applied to the identification of L.monocytogenes. Conclusion Virulence genes are common in strains of L.monocytogenes, and the outbreak of L.monocytogenes infections is potentially threatening. It is required to strengthen the surveillance of L.monocytogenes.更多还原