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高效液相色谱法测定废水中阿维菌素的含量

Determination of Avermectin in Wastewater by High Performance Liquid Chromatography

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【作者】 李再兴宋存义杨景亮邢丽楠郭建博刘英华

【Author】 LI Zai-xing~(1,2),et al(1.University of Science and Technology Beijing,Beijing 100083,China;?2.Hebei University of Science and Technology,Shijiazhuang?050018,China)

【机构】 北京科技大学河北科技大学河北科技大学 北京100083河北石家庄050018北京100083

【摘要】 建立了一种采用高效液相色谱法(HPLC)检测废水中阿维菌素含量的方法。以二氯甲烷作萃取剂萃取废水中的阿维菌素,以甲醇+H2O(88+12)为流动相、Spherisorb C18柱高效液相色谱分离、紫外检测器在波长为245nm时测定。阿维菌素标准曲线的线性回归系数r=0.9948,线性范围为0.025~0.100mg/ml;水样相对标准偏差小于10.6%,加标回收率为75.8%~87.6%。

【Abstract】 Avermectin in wastewater was determined by high performance liquid chromatography.Dichloromethane were used as extractive solvent,and chromatographic separation was performed on a Spherisorb C18 column with methanol/H2O(88+12).The samples was detected by UV detector at 245nm.The linear range of avermectin standard solution was 0.025~0.100mg/ml and the correlation coefficient exceeded 0.9948.The RSD was less than 10.6%.The recovery rate of avermectin wastewater was 75.8%~87.6%.

  • 【文献出处】 中国环境监测 ,Environmental Monitoring in China , 编辑部邮箱 ,2007年03期
  • 【分类号】X832
  • 【被引频次】19
  • 【下载频次】333
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