【摘要】 选择同处于泌乳初期的6头睢宁白山羊为试验动物,于左、右乳区分别灌注黄芪多糖和生理盐水5 mL,连续灌注5 d,第6天注入大肠杆菌内毒素诱导产生山羊实验性乳腺炎。于注入内毒素前1 h及灌注后3、6、9、24 h取颈静脉血和采集乳样,测定乳液及血清中N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)活力及丙二醛含量(MDA)等指标。结果表明:与灌注内毒素前相比,灌注生理盐水的对照乳区乳中的NAGase活力在注入内毒素后9 h时达到峰值(23.95 U/mg),而灌注黄芪多糖的试验乳区乳中的NAGase活力6 h时达到峰值(28.29 U/mg),24 h时乳中NAGase活力已下降到灌注内毒素以前的水平。山羊乳导管注入内毒素后,2种处理的乳区乳中的总抗氧化能力显著下降,乳中MDA的含量显著升高,SOD活力显著下降;2种处理的乳区乳之间比较无显著差异。更多还原

【Abstract】 Six Suining White goats in their early lactation were used in this study.The mammary right quarters of 6 goats were infused with about 5 mL of Astragalus polysaccharide(APS).Left quarters were used as controls and infused with 5 mL of physiological saline solution(PSS).The administration of APS had been continuing for five consecutive days.On the sixth day,after morning milking,the mammary quarters were infused with LPS according to a dosage of 50 μg per kilogram body weight.At varying intervals before and after infusion(-1,3,6,9,and 24 h),quarter milk samples and blood samples from jugular vein were collected.The dynamics of N-acetyl-β-D-glucosaminidase(NAGase) activity,alkaline phosphatase(AKP) activity,SOD activity,and MDA content were determined.The results showed that the NAGase activity in the milk from quarters administered with PSS had reached the peak(23.95 U/mg) at the 9th hour.While the NAGase activity in the milk from quarters administered with APS had reached the peak(28.29 U/mg) at 6th hour and declined to the normal level at 24th hour.Compared to the pre-infusion of LPS,T-AOC in the milk from quarters administered both PSS and APS had decreased significantly,MDA content significantly higher.更多还原