【摘要】 目的研究南极适冷菌Pseudoalteromonas sp.S-15-13胞外多糖EPS-Ⅱ的抑瘤作用。方法不同浓度的EPS-Ⅱ腹腔注射于荷S180肉瘤小鼠,通过荷瘤小鼠的脾重和瘤重变化来测定EPS-Ⅱ的抑瘤效果;采用免疫组化方法,测定荷瘤小鼠增殖细胞核抗原(PCNA),三联组氨酸(FHIT)和S-100蛋白阳性表达的情况。结果(1)不同剂量的EPS-Ⅱ均具有提高荷瘤小鼠脾脏重量,有效抑制肿瘤生长的作用,最高抑瘤率可达45.1%。(2)免疫组织化学结果表明,不同剂量的EPS-Ⅱ均能抑制PCNA表达,促进FHIT和S-100蛋白表达的作用。结论南极适冷菌S-15-13胞外多糖EPS-Ⅱ可能通过调节PCNA,FHIT和S-100的表达来发挥抑瘤作用。更多还原

【Abstract】 Objective To investigate the effects of exopolysaccharides(EPS) purified from an Antarctic psychrotrophs Pseudoalteromonas sp.S-15-13 on the S180 of mice.Methods After different doses of EPS-Ⅱwere injected into abdominal cavity of mice implanted with sarcoma 180 cells for 10 days.The tumor and spleen of mice were weighed and the positive expressions of PCNA,FHIT and S-100 were measured by immunochemistry.Results(1) EPS-Ⅱcould significantly increase the weight of spleen and inhibit the growth of S180 of mice with highest percentage inhibition of 45.1%.(2) Immunohistochemistry analysis showed that EPS-Ⅱcould significantly inhibit the expression of PCNA and enhance the expressions of FHIT and S-100 protein.Conclusion EPS-Ⅱmight inhibit the growth of S180 through modulating the expressions of PCNA,FHIT and S-100.更多还原