【摘要】 将金属盐法与光镜、电镜技术相结合,研究中华绒螯蟹精子形成过程中酸性磷酸酶分布的变化。研究表明,在精子形成的早期,酸性磷酸酶由细胞质中的内质网小泡上产生,随后出现在细胞核内、顶体囊膜上及顶体管内,并且反应产物逐渐由分布均匀的细密颗粒聚集成分布均匀的较大的颗粒;当精子成熟后酸性磷酸酶均匀分布在顶体管中,反应产物颗粒比较大,穿孔器部分发现有少量酸性磷酸酶分布。更多还原

【Abstract】 Ultrastructural cytochemical techniques and electron microscopy were used for localization of acid phosphatase activity during spermiogenesis in Eriocheir sinernsis. The results showed that: Acid phosphatase was synthesized in the endoplasmic reticulum in the early spermatids. The acid phosphatase was found gradually in nucleus,the membrane of acrosomal vesicle,the cytoplasmic region and the acrosomal tubule. And then the reaction product particles became thicker during the spermiogenesis. In the mature sperm,acid phosphatase was localized in the percutor organ slightly,but it was massive and compact in the acrosomal tubule.更多还原