【摘要】 目的为了简化激肽释放酶的制备过程,提高激肽释放酶的纯度、收率和稳定性,建立了一种新的分离纯化工艺。方法直接将胰酶溶解后上DEAE-琼脂糖快胶新型阴离子交换柱吸附,两步洗脱后目标蛋白加入保护剂PEG,经过丙酮低温沉淀,室温干燥即可得到激肽释放酶精品。结果比活力大于550 U.mg-1,收率达73.6%,重复性较好。结论所用工艺可快速分离纯化激肽释放酶。更多还原

【Abstract】 OBJECTIVE A new technology of separation and purification is developed for simplifying the process of extracting of kallikrein and increasing the purity and yield of kallikrein.METHODS The crude extraction from the dissolution of pancreatin was directly absorbed on the DEAE gelose fast flow column which is a new type of anion exchange chromatographic column,and then eluted by two steps to acquire the target protein.The target protein was protected by PEG and then precipitated by acetone at low temperature.Fine kallikrein was obtained by being desiccated at room temperature.RESULTS The specific activity was more than 550 U·mg-1,the yield was 73.6%,and the recurrence was good.CONCLUSION This technology can separate and purify kallikrein rapidly.更多还原