【摘要】 目的探讨肝细胞生长因子(HGF)在大鼠修复性牙本质形成过程中的作用。方法以窝洞预备形成大鼠修复性牙本质模型,免疫组织化学染色法检测HGF在窝洞预备后3 d、15 d、30 d的大鼠牙髓组织中的表达,采用积分光密度值(IOD)定量修复性牙本质形成不同阶段HGF的表达。结果窝洞预备后3 d,HGF在大鼠牙髓细胞及成牙本质细胞胞浆中呈强阳性表达(IOD为8.995±0.943);窝洞预备后15 d,HGF在两种细胞的表达仍呈阳性(IOD为5.624±0.951),但弱于3 d组(P<0.01);30 d组(4.073±0.127)和正常对照组(4.279±0.348)大鼠牙髓细胞及成牙本质细胞胞浆中HGF均呈弱阳性表达,两者间差异无统计学意义。结论HGF参与了牙髓损伤早期修复及修复性牙本质形成的过程。更多还原

【Abstract】 Objective To investigate the role of hepatocyte growth factor(HGF) in rat’s reparative dentinogenesis.Methods Reparative dentinogenesis was induced by preparing cavity on the first molar of SD rat.The teeth were collected on the 3rd,15th,30th day after the preparation of cavity,respectively.The healthy pulp was used as control.The expression of HGF in different stages of reparative dentinogenesis was detected by immunohistochemical method.The levels of HGF expression were quantitated by measurement of integral optical density(IOD).Results On the 3th day after cavity preparation,strong expression of HGF in the odontoblast layers and the cytoplasts of dental pulp cells was noted;the IOD value was 8.995±0.943.On the 15th day,positive expression of HGF was observed,the IOD value(5.624±0.951) was lower than that on the 3th day(P<0.01).On the 30th day,the HGF expression(IOD 4.073±0.127) was found to be weak and similar to the control(IOD 4.279±0.348)(P>0.05).Conclusion HGF may play an important role in the early stages of dental pulp repair.更多还原