【摘要】 目的研究马桑内酯(coriaria lactone,CL)对大鼠海马神经元钠离子通道电流的影响,探讨该影响在CL致痫中的意义。方法利用全细胞膜片钳技术,在急性分离的大鼠海马神经元上记录钠电流,观察CL对电流幅度的影响。结果经0.1 m g/mL、0.2 m g/mL的CL作用后,海马神经元钠电流有不同程度的增加〔CL 0.1 m g/mL组的最大峰值电流密度为(-90.11±14.02)pA/pF,增幅为17.32%±8.52%;CL 0.2 m g/mL组的最大峰值电流密度为(-111.52±6.65)pA/pF,增幅为37.98%±4.91%〕;经与对照组相比,CL 0.2 m g/mL组(P<0.05)的变化较CL 0.1 m g/mL组(P>0.05)明显。结论CL使海马神经元电压依赖性钠电流的幅度增大,从而改变细胞的兴奋性,引发异常放电。更多还原

【Abstract】 Objective The present study tried to find out the effects of coriaria lactone(CL) on the sodium currents of the rat hippocampal neurons and their significance.Methods The sodium currents of the acutely isolated Sprague-Dawley rat hippocampal neurons were recorded by employing the patch-clamp technique in the whole-cell voltage-clamp mode.The effects of CL on the peak currents in the neuron membrane were assessed and analyzed.Results Both 0.1 mg/mL and 0.2 mg/mL CL increased the maximal peak density of sodium currents significantly(17.32%±8.52% vs 37.98%±4.91%).The effect of 0.2 mg/mL CL(P<0.05) was more significant than that of 0.1 mg/mL CL(P>0.05),when compared with the control(3.16%±0.65%).Conclusion CL can increase the amplitudes of the voltage-dependent sodium currents in the acutely isolated hippocampal neurons.This effect may contribute to the enhanced excitability of hippocampal neurons and play a role in the epileptogenetic process of CL.更多还原