【摘要】 目的探讨乳腺癌组织中缺氧诱导因子-1α(hypox ia-induc ib le factor 1 a lpha,H IF-1α)的表达及其与增殖细胞核抗原(pro liferating ce ll nuclear an tigen,PCNA)、血管内皮生长因子(vascu lar endothe lia l grow th fac-tor,VEGF)及临床病理因素的关系。方法采用免疫组化SP法检测乳腺纤维腺瘤、普通乳腺增生组织和乳腺癌中H IF-1α、PCNA、VEGF蛋白的表达。结果H IF-1α蛋白在乳腺纤维腺瘤、普通乳腺增生组织中不表达;乳腺导管内原位癌中阳性率55%(11/20),浸润性乳腺癌中85%(51/60);乳腺癌中VEGF阳性率81.3%(65/80);乳腺癌中PCNA阳性率75%(60/80),其中原位癌中65%(13/20),浸润癌中78.3%(47/60)。乳腺癌中H IF-1α表达与VEGF(r=0.762,P<0.01)、PCNA(r=0.693,P<0.01)均呈正相关;H IF-1α在淋巴结转移与否、不同雌激素受体状态及组织学分级中表达的差异有统计学意义。结论H IF-1α及其靶基因VEGF在乳腺癌组织中明显上调,H IF-1α表达与肿瘤细胞增殖、淋巴结转移、雌激素受体状态及组织学分级相关,提示H IF-1α对乳腺癌的发生发展起重要作用,有望成为肿瘤放、化疗及生物治疗的一个新靶点。更多还原

【Abstract】 Objective To investigate the expression of hypoxia-inducible 1 alpha(HIF-1α) in human breast cancer and its relationship with vascular endothelial growth factor(VEGF) protein,proliferating cell nuclear antigen(PCNA) protein,other tumor biomarkers and clinical pathologic factors.Methods The immunohistochemical staining(SP) was used to measure the expression of HIF-1α,VEGF and PCNA in human breast fibroadenoma,usual hyperplasia and breast carcinoma.Results HIF-1α was not found expressing in breast fibroadenoma and hyperplastic lesions.In contrast,the positive rate of HIF-1α was found in the ductal carcinoma in situ 55%(DCIS,11/20) and the invasive breast carcinoma 85%(51/60).VEGF positivity in breast carcinoma was 81.3%(65/80).The total positive rate of PCNA in breast carcinoma was 75%(60/80),that in DCIS was 65%(13/20) and that in invasive carcinoma was 78.3%(47/60).Increased levels of HIF-1α were statistically significantly associated with increased expression of VEGF(r=0.762,P<0.01) and PCNA(r=0.693,P<0.01).Conclusion The upregulated expression of HIF-1α and VEGF in breast carcinoma has a close relationship with tumor angiogenesis,tumor cell proliferation,lymph node metastasis,ER status and stages of histology.This suggests that HIF-1α plays an important role in the carcinogenesis and progression of breast carcinoma;is wished to become a new target for radiotherapy,chemotherapy and biotherapy of tumor,which will offer the new ways to diagnosis and treatment of tumor.更多还原