【摘要】 利用伴刀豆球蛋白A和糖类的特异性相互作用,研制了葡萄糖表面等离子体共振传感器.传感器的敏感膜是构建于金膜表面的伴刀豆球蛋白A/葡聚糖自组装多层膜.在葡萄糖的存在下,该自组装多层膜被分解,引起表面等离子体共振信号的显著变化,信号变化的大小与葡萄糖的浓度相关.结果表明,利用该传感器可以选择性地检测0.1～50mmol·L-1浓度范围内的葡萄糖,且敏感膜可以多次再生使用.更多还原

【Abstract】 A glucose-selective surface plasmon resonance sensor was constructed by using specific interaction between concanavalin A and sugars. The sensing element was self-assembled multilayers of concanavalin A and dextran formed on gold film. In the presence of glucose, the self-assembled multilayers were disintegrated. As a result, the surface plasmon resonance signal was changed evidently. The signal changes were correlative to the concentration of glucose. The results showed that this sensor could detect the glucose selectively in the range of 0.1～50 mmol·L-1, and the sensing element could be regenerated for several times.更多还原