【摘要】 目的观察重组质粒pIRES-fimA:IL15经滴鼻免疫BABL/c小鼠后诱导产生的血清和唾液抗体反应及白细胞介素-15(IL-15)对sIgA反应的调节作用。方法重组质粒pIRES-fimA:IL15与pIRES-fimA经滴鼻免疫和肌肉注射免疫BABL/c小鼠,以间接ELISA方法检测血清中IgG和唾液中sIgA抗体水平。结果重组质粒pIRES-fimA:IL15与pIRES-fimA经滴鼻免疫可诱导产生唾液sIgA反应,且该反应明显高于肌肉注射组,血清IgG水平与肌肉注射组无统计学差异。pIRES-fimA:IL15经滴鼻免疫产生的唾液sIgA滴度显著高于pIRES-fimA组,且有统计学差异(P<0.05)。结论滴鼻免疫可以作为抗牙龈卟啉单胞菌DNA疫苗有效的黏膜免疫途径,诱导循环和口腔局部抗体反应;IL-15可以作为细胞因子佐剂通过滴鼻途径增强该疫苗诱导的sIgA反应强度。更多还原

【Abstract】 Objective To observe the antibody responses induced by recombinant plasmid pIRES-fimA:IL15 via nasal immunization to BABL/c mice and the regulation of IL-15 to sIgA.Methods BABL/c mice were immunized with recombinant plasmids pIRES-fimA:IL15 and pIRES-fimA via nasal or intramuscular route.Serum IgG and salivary sIgA levels after immunization were analyzed by ELISA.Results Nasal immunization with plasmids pIRES-fimA:IL15 or pIRES-fimA elicited significant higher level of salivary FimA-specific sIgA responses compared with intramuscular immunization.There was no significant difference of the serum IgG responses between nasal immuni-zation mice and intramuscular immunization mice.Nasal immunization with plasmid pIRES-fimA:IL15 elicited significant higher level of salivary sIgA response than with pIRES-fimA(P<0.05).Conclusion Nasal dropping may be an effective mucosal immunization route of anti-Porphyromonas gingivalis DNA vaccine to elicit specific antibody responses in serum and oral region.IL-15 has a positive regulation effect to sIgA response.更多还原