【摘要】 用腺病毒重组体(AdCMV-p53/GFP)转染经0.5,1.0和2.0Gyγ射线辐射处理的前列腺癌细胞[PC-3(nullp53)],用克隆形成法检测细胞增殖能力,用流式细胞分析法测定腺病毒重组体转染率和外源性p53蛋白表达。结果提示,辐射诱导使腺病毒重组体转染PC-3细胞提高7%—39%。辐射联合AdCMV-p53转染组p53表达水平提高18.5%—35.4%。与单纯AdCMV-p53转染组和单纯辐射组相比,辐射联合AdCMV-p53转染组细胞存活率分别降低25%—64%和22%—65%。更多还原

【Abstract】 To determine whether low-dose pre-irradiation could enhance adenovirus-mediated p53 transfer and expression in human prostate adenocarcinoma, the PC-3 cells were pre-exposed to γ rays, and then infected with replication-deficient adenovirus recombinant vectors, containing human wild-type p53 (AdCMV-p53) or green fluorescent protein gene (AdCMV-GFP) respectively (γ-ray irradiation + AdCMV-p53 /GFP infection). The exogenous gene transfer and expression were detected by flow cytometric analysis. The GFP transfer frequencies in γ irradiation + AdCMV-GFP infection groups were 7%—39% more than those in AdCMV-GFP infection groups. The p53 levels in the γ irradiation + AdCMV-p53 infection groups were 18.5%—35.4% more than those in AdCMV-p53 infection groups (p<0.05), suggesting that low-dose (less than or equal to 1.0 Gy) irradiation could significantly promote exogenous p53 transfer and expression in the PC-3 cells. The survival fractions for the γ irradiation + AdCMV-p53 infection groups were 25%—64%, 22%—65% less than those for AdCMV-p53 infection, or γ-irradiation groups, respectively (p<0.05).更多还原