【摘要】 目的观察助孕胶囊对多囊卵巢(PCO)大鼠生殖内分泌激素、卵巢组织肿瘤坏死因子α表达的影响,探讨其对PCO模型大鼠可能的作用机制。方法采用孕激素联合人绒毛膜促性腺激素(HCG)造模法制备PCO模型大鼠。随机分为对照组、PCO模型组(模型组)、PCO模型组+助孕胶囊组(中药组)、PCO模型组+克罗米酚组(西药组),运用放免法测定血清性激素水平的变化;光镜观察卵巢、卵泡发育的影响,免疫组化学法检测卵巢组织肿瘤坏死因子α表达水平。结果助孕胶囊可使PCO大鼠的颗粒细胞层增厚,卵泡膜细胞层变薄,血清T、LH水平均明显下降,并下调卵巢组织肿瘤坏死因子α的表达。结论助孕胶囊能够通过调整内分泌及影响卵巢肿瘤坏死因子α的表达,在中枢及卵巢局部协调发挥作用,从而调整性腺功能,促进卵泡的发育成熟及排卵。更多还原

【Abstract】 Objective To observe the effect of Zhuyun capsule(ZYC) on expression of genital endocrine hormone and tumor necrosis factor-alpha(TNF-α) in PCO rats,and investigate therapeutic actions and possible therapeutic mechanism of the capsule.Methods The PCO rat models,prepared by pregnancy hormone combine with human chorionic gonadotrophin(HCG),randomly divided into four group as control,model,Chinese crude drug(CCDG) and western medicine group(WMG);The levels of serum gonadal hormone were detected by radioimmunoassay;The effects of ZYC on ovaries and ovarial follicular development were observed by light microscope;And the expression level of TNF-α was detected with the immunohistochemical staining technique.Results ZYC could increase the thickness of granular cell layer and decrease the thickness of theca cell layer,greatly decrease the level of T and LH in serum and the expression of TNF-α in rat ovary.Conclusion ZYC can function regulating the endocrine and affecting the expression of the TNF-α coherently in center and ovary to adjust genital gland and promote the mature and ovulation of follicular.更多还原