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神经型一氧化氮合酶对脑损伤后神经细胞凋亡的影响

Effect of nNOS on the neuronal apoptosis after traumatic brain injury in rats

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【作者】 高广伟刘云贺汪汉民

【Author】 GAO Guangwei,LIU Yunhe,WANG Hanmin.Department of Neurosurgery, the Third People’s Hospital of Luohe,Luohe 462000,China

【机构】 漯河市第三人民医院神经外科漯河市第三人民医院神经外科 河南省462000河南省462000

【摘要】 目的:探讨脑损伤后神经型一氧化氮合酶(nN 0S)对神经细胞凋亡的影响及其相关机制。方法:180只SD大鼠随机分成以下三组:假手术组、脑创伤组、7-硝基吲唑(7-N I)组,此三组分别划分为伤后3,6,12,24,48,72 h六个时相组,每个时相组均为10只大鼠,采用M arm arou法制造大鼠重型弥漫性颅脑创伤模型,运用原位末瑞标记TUNEL法和免疫组化法,观察三组不同时相点海马CA 1区的神经细胞凋亡情况和B cl-2的表达情况。结果:(1)假手术组偶见TUNEL阳性凋亡细胞及B cl-2阳性细胞。(2)脑创伤组,伤后各时相点均出现TUNEL阳性凋亡细胞及B cl-2阳性细胞,先上升后下降。(3)7-N I组,伤后6,12,24 h凋亡细胞明显下降而B cl-2阳性细胞却明显上升(同脑创伤组比较P<0.05)。结论:在脑损伤的早期,nNO S能够通过抑制B cl-2的表达来促进神经细胞的凋亡。

【Abstract】 Objective To investigate the effects of nNOS on the neuronal apoptosis after traumatic brain injury and the related mechanism.Methods One hundred and eighty male SD rats were randomly divided into 3 groups: fake operation group,trauma group,7-NI(the inhibitor of nNOS)group.The groups were divided into 6 subgroups according to the time phase after brain injury,which was 3 h,6 h,12 h,24 h,48 h and 72 h respectively.Each subgroup had 10 rats.Marmarou model of severe brain injury in rats were used to investigate the neuronal apoptosis,the expression of Bcl-2 in the hippocampal CA1 region of each time point after brain injury by TUNEL situ and immunohistochemistry.Results ① In fake operation,TUNEL positive cells and Bcl-2 positive cells could be observed occasionally.②In trauma group,TUNEL positive cells and Bcl-2 positive cells could be observed at each time point after injury.③In 7-NI group,the number of TUNEL positive cells at 6,12,and 24 h after injury was decreased significantly in comparison with that of trauma group(P<0.05),but Bcl-2 positive cells increased significantly.Conclusion nNOS can promote the neuronal apoptosis by inhibiting the expression of bcl-2 during the earlier period after brain injury.

【关键词】 创伤性脑损伤凋亡一氧化氮合酶Bcl-2
【Key words】 Traumatic brain injuryapoptosisNOSBcl-2
  • 【文献出处】 实用诊断与治疗杂志 ,Journal of Practical Diagnosis and Therapy , 编辑部邮箱 ,2007年01期
  • 【分类号】R651
  • 【被引频次】10
  • 【下载频次】224
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