【摘要】 【目的】多发性硬化(Multiple Sclerosis,MS)发病机制同T细胞异常活化介导的免疫紊乱密切相关。而Th1/Th2类细胞因子的失衡被认为积极参与了多发性硬化的病变过程。本文采集了多发性硬化病人在甲强龙冲击前后的外周血单核细胞(PBMC)进行体外培养,考察其在病情急性期及缓解期Th1/Th2类因子的分泌情况。【方法】收集多发性硬化病人急性期及缓解期外周血PBMC进行体外培养,应用ELISA法检测PBMC培养上清IFN-γ和IL-10含量,同对照健康患者进行比较,并对MS患者治疗前后进行自身对照比较。【结果】14名多发性硬化患者经甲强龙冲击后12名患者有明显或一定程度缓解,MS患者治疗前PBMC上清中IFN-γ含量显著高于对照组(P<0.05),治疗后MS患者同治疗前比较其PBMC分泌IFN-γ能力显著下降(P<0.05)。【结论】Th1类细胞因子积极参与了多发性硬化的发病过程,甲强龙冲击治疗后降低了Th1类细胞因子的分泌,使病情趋向缓解。更多还原

【Abstract】 【Objective】The pathogenesis of Multiple Sclerosis(MS) is closely related to the abnormal activation of T cell and the imbalance of Th1/Th2 has been considered to be involved in.The PBMCs of MS patients before and after Methylprednisolone(MP) treatment were collected to detect the secretion of IFN-γ and IL-10 from PBMC.【Methods】PBMCs from MS patients at acute or remission stage were collected respectively,ELISA was adopted to detect IFN-γand IL-10 in the supernatant of PBMC,and then the comparisons of cytokines between MS patients and control,and the comparisons between the same MS patients before and after MP treatment were made.【Results】After the MP treatment of all 14 MS patients,12 of which evolved into remission stages,the IFN-γof PBMC from MS patients before MP treatment was significantly higher than control(P<0.05),the IFN-γof PBMC from MS patients after MP treatment was significantly lower than that of MS patients before MP treatment(P<0.05).【Conclusion】Th1 cyokines is actively involved in the pathogenesis of MS,and MP treatment reduces the Th1 secretion significantly which promotes the remission of MS.更多还原