【摘要】 【目的】探讨灯盏细辛(EBHM)对氧致视网膜病变(OIR)的疗效。【方法】36只C57BL/6J幼鼠随机分为A、B、C三组。A和C组制备OIR动物模型。A组腹腔注射灯盏细辛,小剂量10 mg/0.2 ml为AⅠ组,大剂量20 mg/0.2 ml为AⅡ组。C组注射磷酸缓冲盐溶液(PBS)。B组为正常氧环境生长的对照组。幼鼠均行眼切片新生血管芽细胞核计数及视网膜铺片计分。【结果】B组眼切片新生血管芽细胞核计数及视网膜铺片计分与C组相比,差异显著(均P<0.01)。灯盏细辛治疗后芽细胞核计数及视网膜铺片计分AⅡ组与C组相比均有明显差异(均P<0.01),AⅠ组与C组无明显差异(均P>0.05)。【结论】氧能诱导幼鼠产生视网膜新生血管病变。大剂量灯盏细辛能促进视网膜新生血管部分萎缩,有效治疗OIR。更多还原

【Abstract】 【Objective】To assess the effect of erigeron breviscapus(vant) hand-mazz(EBHM) on the mouse model with oxygen induced retinopathy(OIR).【Methods】Thirty-six 7-day-old(postnatal day 7,P7) C57BL/6J mice were divided into three groups: group A,group B and group C.Mice in group B lived in the environment with normal oxygen.Mouse models of oxygen induced retinopathy in group A and group C were used to produce retinal neovascularization.EBHM[10 mg/(0.2 ml·day) to group AⅠand 20 mg/(0.2ml·day) to group AⅡ]was intraperitoneally administered to group A of OIR to determine if it cured retinopathy.PBS was used in group C.Fluorescein conjugated dextran angiography of retinal vasculature was employed and retinal whole mounts were repared to score features of the retinopathy.In addition,quantification of retinal neovascular nuclei was calculated on the sections of HE staining.【Results】Retinopathy score of animals in group C was higher than that of control animals(group B)(P<0.01).In addition,quantification of neovascular nuclei showed a significant increase in the number of nuclei extending beyond the inner limiting membrane into the vitreous in group C(P<0.01).The average number of extraretinal neovascular nuclei on six retinal sections and retinopathy score were significantly decreased by EBHM at doses of 20 mg/(0.2ml·day) when compared to group C(P<0.01).【Conclusion】EBHM can cure oxyen induced retinopathy in the mouse.The proliferation of retinal new vessel may be suppressed by EBHM partially.更多还原