【摘要】 目的:对比两种方法检测感染家兔血液中弓形虫基因动态检测结果。方法:用RH株弓形虫感染雄性家兔16只,在感染前后耳缘静脉采集血液标本。用荧光定量PCR(Fluorescence Quantitiative PCR,FQ-PCR)和普通PCR分别检测血液标本中虫体DNA基因片段。结果:FQ-PCR检测,其总阳性率为64.42%。感染家兔血液中虫体密度高峰期在感染后1～3 wk左右,其后长期维持在较低水平;普通PCR检测感染家兔总阳性率为45%。感染后第1wk检出率仅22%,高峰期维持在感染后2～4 wk,检出率约为60%左右。其后检出率下降为25%左右并一直维持。结论:两种PCR检测方法有相同的动态检出效果,采用血液作为样品进行弓形虫PCR检测时其最佳检测时间为感染后1～4 wk,其后检出率逐渐下降。更多还原

【Abstract】 Objective: To compare the dynamic detections of Toxoplasma gondii(T.g) DNA in blood and semen from infected rabbits by two PCR.Methods: 16 rabbitts were infected with T.g tachyzoites by intraperitoneal injection.The samples of blood were collected from the rabbit’s helix vein for later detection.The FQ-PCR and conventional PCR were used to detect the T.g DNA.Results: The results of detection of T.g DNA in blood from infected rabbits by two PCR were continuously compared.The positive rate of all blood samples of infected rabbits by FQ-PCR is 64.42%.The peak of density of T.g in blood appeared in 1～3 week after infection.After 3 weeks the density of T.g was kept low level.That by conventional PCR was 45%.The positive rate of rabbits 1 week after infection by this PCR only was 22%.The peak of positive rate by this PCR were about 60% and kept 2～4 week after infection.After that time the positive rate was maintained about 22%.Conclusion: There was the similar results of dynamic detections of T.g DNA between the two PCR.The optimal time of detection of T.g DNA in blood by PCR was 1～4 week after infection.After that time the detection rate was fall off.更多还原