【摘要】 目的:观察清肝胶囊对小鼠急性肝损伤的改善作用。方法:健康昆明种小鼠50只,随机分为5组:正常对照组,模型对照组,清肝胶囊低剂量组、中剂量组、高剂量组,每组10只。先以羧甲基纤维素钠20mL/(kg.d)、清肝胶囊0.5g/(kg.d)、1.0g/(kg.d)、1.5g/(kg.d)经口灌胃给药,1次/d,连用7d,于最后1次给药后30min,除正常对照组外,其他4组均采用D-氨基半乳糖700mg/kg腹腔注射造成小鼠急性肝损伤模型,24h后处理动物,测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、细胞色素P450(CYP450)含量及观察肝脏病理学的改变情况。结果:中高清肝胶囊剂量组与模型对照组相比血清中ALT、AST明显降低(P<0.05),CYP450含量明显升高(P<0.05),肝细胞变性、坏死明显减轻。结论:中高剂量的清肝胶囊对由D-氨基半乳糖所致的急性肝损伤有明显的保护作用,其机制可能与CYP450表达增加有关。更多还原

【Abstract】 Aim:To observe the protective effects of Qinggan capsule(QGC) on acute liver injury in mice.Methods:Fifty healthy KM mice were randomly divided into five groups:normal control group,D-galactosamine(D-GalN) model control group and QGC low-,middle-,high-dose groups,10 mice in each group.The mice were given sodium carboxy methyl cellulose(CMC-Na) at 20 mL/(kg·d) and QGJN at different doses(0.5 g/(kg·d),1.0 g/(kg·d) and 1.5 g/(kg·d) intragasticly,one time per day for 7 days.Except the normal control group,the other 4 groups were given D-GalN intraperitioneally 30 min after last administration to make the model of acute liver injury.Determinations of serum ALT,AST,cytochrome P450(CYP450) and pathological examination were carried out in all the mice after 24 h.Results:Compared with the model control group,the serum levels of ALT and AST remarkably decreased(P<0.05),and the content of CYP450 notably increased(P<0.05) in QGC middle-and high-dose groups,and the degeneration and necrosis of liver cells were also significantly alleviated.Conclusion:QGC has protective effects on acute liver injury induced by D-GalN,and the mechanism may be related to QGC increasing the expression of CYP450.更多还原