【摘要】 目的:观察盐酸山莨菪碱(654-2)对豚鼠噪声性听力损伤的保护作用。方法:30只豚鼠随机分为对照组、损伤组与654-2防治组。损伤组与654-2防治组均给予噪声暴露。654-2防治组在噪声暴露前30min肌内注射654-2,另2组同法注射生理盐水。各组豚鼠在噪声暴露前与暴露后3d、14d、28d分别测ABR阈值,并采用硫代巴比妥钠荧光比色法测定耳蜗MDA含量。结果:噪声暴露后3d、14d、28d损伤组和防治组ABR阈移与耳蜗MDA含量均较对照组升高(P<0.01);654-2防治组噪声暴露后14d与28d时,以上2指标均低于损伤组(P<0.05或0.01)。结论:654-2可部分减轻噪声所致的听力损失。其机制可能为654-2在一定程度上清除了噪声产生的过量过氧化物从而保护了毛细胞,使听力得到部分恢复。更多还原

【Abstract】 Aim:To observe the protective effect of anisodamine on the guinea pigs after noise exposure.Methods:A total of 28 guinea pigs were allocated into three groups:the control group,the damaged group,and the treatment group(654-2 group).The latter 2 groups were given noise exposure and the 654-2 group was pretreated with 654-2 at 30 min before noise exposure.Thio-barbital sodium fluorimetry method was used to detect the content of MDA,and the hearing threshold of ABR on the 3rd day,14th day,and 28th day after noise exposure was also detected.Results:On the 3rd day,14th day,and 28th day after noise exposure,ABR threshold shift and the cotents of MDA in damaged group and 654-2 group were higher than those in control group(P<0.01);on the 14th day and 28th day after noise exposure,the above 2 indexes in 654-2 group were lower than those in damaged group(P<0.05 or 0.01).Conclusion:654-2 can prevent and treat noise-induced hearing damage in a certain degree,which may result from the 654-2 protecting hair cells through cleaning excess peroxides after noise exposure.更多还原