【摘要】 目的:应用高效液相色谱法(HPLC)-电化学法检测人血浆中盐酸克仑特罗含量。方法:采用乙醚-乙醇混合液提取,用C18色谱柱,流动相为体积比7:3的0.02mol/L磷酸二氢钾-乙腈混合液,检测器为电化学检测器,电位0.80V,内标法定量。结果:本法的线性范围是1.5~120.0μg/L,回归方程Y=0.753X+0.011,r=0.9993;最低检出限0.015μg/L,RSD为2.6%~4.2%,平均回收率为93.8%。结论:本法具有灵敏、准确、快速、稳定的特点。更多还原

【Abstract】 Aim:To detect clenbuterol-hydrochloride in human plasma by using high performance liquid chromatography(HPLC)/ECD method.Methods:The assay was based on internal standard with hydroquinone, a nucleodur C18 column, phosphate buffer and acetonitrile(7:3) as mobile phase with flow rate of 1.2 mL/min. The potential of working electric was maintained 0.8 V. Results: The linear range was 1.5～120.0 μg/L (Y=0.753X+0.011,r=0.999 3).Recovery rate of component added to plasma samples was 93.4%. The minimal detection limit was 0.015 μg/L. RSD was 2.6%～4.2%.Conclusion: HPLC/ECD method has many advantages such as rapidity, simplicity, accuracy, sensitivity, and higher distinction.更多还原