【摘要】 目的:探讨心肌梗死后凋亡基因PDCD5表达的变化及阿托伐他汀对其表达的影响。方法:结扎雄性Wistar大鼠左冠状动脉前降支造成心肌梗死(MI)模型。取大鼠45只,其中30只MI后24h随机分为MI组(n=15)和阿托伐他汀组(n=15);余15只作为假手术组。阿托伐他汀组术后每d灌胃阿托伐他汀1mg/kg,余2组灌胃等量生理盐水。6周后检测左心室非梗死区心肌组织中肿瘤坏死因子α(TNF-α)的含量、凋亡基因PDCD5mR-NA及其蛋白产物的表达。结果:6周后MI组、阿托伐他汀组和假手术组存活大鼠分别为10只、11只和14只。MI组非梗死区心肌组织TNF-α含量和PDCD5mRNA及PDCD5蛋白表达高于假手术组(P均<0.05);阿托伐他汀组上述指标的表达高于假手术组(P均<0.05),但均低于MI组(P<0.05)。结论:阿托伐他汀可能通过降低大鼠MI后非梗死区心肌组织TNF-α的含量而降低非梗死区心肌组织中PDCD5mRNA及其蛋白的表达。更多还原

【Abstract】 Aim:To study the effect of atorvastatin on the expression of TNF-α and PDCD5 after myocardial infarction(MI)in rats.Methods:A total of 30 rats,subjected to make MI animal model through ligating the left anterior descending coronary of rats,were randomly divided into 2 groups:MI group(n=15)without any treatment and atorvastatin group(n=15)treated with atorvastatin 1 mg/kg.15 rats were the sham operation group(n=15).TNF-α,the mRNA and protein of PDCD5 in the non-infarted myocardial tissue were detected by RT-PCR 6 weeks after treatment.Results:6 weeks later,there were 10,11,and 14 rats survived in MI group,atorvastatin group and sham operation group,respectively.The expression of TNF-α,the mRNA and protein of PDCD5 in the non-infarted myocardial tissue of atorvastatin group were higher(P<0.05)than those of the sham operation group,but lower than those of MI group(P<0.05).Conclusion:Atorvastatin decreases the expression of mRNA and protein of PDCD5 through inpressing the expression of TNF-α in the non-infarted myocardial tissue.更多还原