【摘要】 目的:探讨调控Livin基因表达对食管癌EC9706细胞生长速度、细胞凋亡以及放射线敏感性的影响。方法:取EC9706细胞,分7组,siRNA组、Livinα组、Livinβ组分别用脂质体包裹法转染沉默Livin表达的siRNA载体pSUPER-neo-Sli1、Livinα表达载体pIRES2-AcGFP-Livinα及Livinβ表达载体pIRES2-AcGFP-Livinβ;Livinα/β组同时转染pIRES2-AcGFP-Livinα和pIRES2-AcGFP-Livinβ,空siRNA载体组转染pSUPER-neo,空表达载体组转染pIRES2-AcGFP,空白对照组不转染。取各组细胞,荧光定量RT-PCR法检测Livin mRNA表达情况,MTT法测定细胞生长曲线,TUNEL法检测细胞凋亡,同时检测Caspase-3活性及放射线敏感性。结果:与空白对照组比较,空表达载体组、空siRNA载体组细胞倍增时间、凋亡指数、Caspase-3活性和放射线敏感性差异均无统计学意义(P>0.05)。与空白对照组比较,Livinα组、Livinβ组和Livinα/β组细胞倍增时间、凋亡指数、Caspase-3活性和放射线敏感性均降低(P<0.05);siRNA组细胞凋亡指数、Caspase-3活性和放射线敏感性增加(P<0.05),细胞倍增时间差异无统计学意义(P>0.05)。结论:调控EC9706细胞Livin基因的表达,可有效改变细胞的生物学行为;Livin基因可能成为肿瘤治疗的新靶点。更多还原

【Abstract】 Aim:To observe the influence of regulating Livin gene expression on esophageal carcinoma cell line EC9706 growth,cell apoptosis and sensitivity to radioactive rays.Methods:EC9706 were divided into 7 groups and transfected different plasmids using packaged by liposome,respectively.siRNA group was transfected pSUPER-neo-Sli1vector which can silence the expression of Livin gene,Livinα and Livinβ groups were transfected Livin expression vector pIRES2-AcGFP-Livinα and pIRES2-AcGFP-Livinβ,respectively,Livinα/β group was transfected the two expression vector,and the control group A and B were transfected pSUPER-neo,pIRES2-AcGFP,respectively.The Livin mRNA,growth curve,Caspase-3 activity,apoptosis and sensitivity to radioactive rays of cells with and without transfection were detected.Results:Compared with cells without transfection,population doubling time,apoptosis rate,Caspase-3 activity and sensitivity to radioactive rays of cells in Livinα,Livinβ and Livinα/β groups decreased significantly(P<0.05);while those of cells in siRNA group increased significantly(P>0.05).Conclusion:Regulating Livin gene expression can change the EC9706 cells biological behavior effectively.Livin gene may be a promising molecule target in the tumor gene therapy.更多还原