【摘要】 目的建立一种直接测定血红蛋白的新方法。方法利用表面活性剂十六烷基三甲基溴化铵(CTAB)和Zn2+对血红蛋白(hemoglobin,Hb)的荧光强度有显著的增强作用,用荧光光谱法对人血清中的血红蛋白进行定量测定。结果方法的线性范围为2×10-8~6×10-7mol/L,检出限为1.9×10-9mol/L,加标回收率99.10%~99.61%。工作曲线的相关系数为0.9994。结论该方法简单,快速灵敏,测试成本低,结果满意。更多还原

【Abstract】 Aim: To establish a new molecular fluorescence spectroscopy for the determination of hemoglobin(Hb) directly.Methods:Because of the surfactant hexadecyl trimethyl ammonium bromide (CTAB) and zinc-ion had the strong enhancement on the fluorescence of Hb, the fluorescence intensity of Hb in human serum was measured using fluorescence spectrophotometer. Results: The linear range of Hb was 2×10-8～6×10-7 mol/L, the detection limit was 1.9×10-9 mol/L, and the recovery ratio was 99.10 %~99.61%. The regression coefficient of the curve was 0.999 4. Conclusion: The method is simple, accurate and quick with low price.更多还原