【摘要】 目的构建表达N端2-8位氨基酸缺失的突变型单核细胞趋化蛋白-1(mMCP-1),并观察其对单核细胞趋化蛋白-1(MCP-1)的干预作用。方法Megaprimer-PCR构建mMCP-1-pVAX1(pVMm)。以脂质体将pVMm转染真核细胞系C2C12,ELISA分析mMCP-1的表达情况。采用趋化实验分析mMCP-1对病毒性心肌炎小鼠外周血单个核细胞(PMNCs)的趋化活性及其对MCP-1的干预作用。结果①酶切和测序显示野生型MCP-1真核表达载体(pVM)和pVMm构建成功。pVMm在真核细胞C2C12中有效表达。②mMCP-1不具有趋化病毒性心肌炎小鼠PMNCs的活性。与单纯使用MCP-1相比,以不同浓度的mMCP-1和MCP-1同时行趋化实验,被趋化的细胞明显减少(P<0.01),25μg/LmMCP-1可阻断10μg/LMCP-1对病毒性心肌炎小鼠PMNC的趋化活性。结论小鼠MCP-1N端2-8位氨基酸决定其趋化活性。mMCP-1可用于干预MCP-1的作用。更多还原

【Abstract】 Aim: To construct and express N-terminus deleted monocyte chemoattractant protein-1(mutated MCP-1,mMCP-1) and observe its biological activity.Methods:The plasmid pVMm carrying murine mMCP-1 coding gene was constructed by Megaprimer-PCR. Lipofectamine was used to transfect pVMm into C2C12 and the expression of mMCP-1 was detected by ELISA. Chemoattractant activity of mMCP-1 to peripheral blood mononuclear cells(PMNCs) of mice with viral myocarditis and the intervention of mMCP-1 with the role of MCP-1 were analyzed by chemotaxis assay. Results: pVM carrying murine wild-type MCP-1 coding gene and pVMm were successfully constructed according to enzyme digestion and sequencing. mMCP-1 was expressed by C2C12 after transfected with pVMm. mMCP-1 was not able to chemoattract the PMNC s of mice with viral myocarditis. Compared with MCP-1 alone, the chemoattractant cells were reduced by different concentrations of mMCP-1 and 10 μg/L MCP-1(P<0.01). 25 μg/L mMCP-1 could block the chemoattractant activity of 10 μg/L MCP-1 to PMNCs of mice with viral myocarditis. Conclusion: N-terminus 2-8 amino acid of murine MCP-1 is the region with chemoattract activity. mMCP-1 could interfere with the role of MCP-1.更多还原