【摘要】 目的:探讨不同剂量的锌对高氟所致雄性大鼠生殖损伤的拮抗作用。方法:将50只雄性Wistar大鼠随机分为5组,即对照组(去离子水)、氟组(25mg/kg氟化钠)、氟加低锌组(25mg/kg氟化钠+4mg/kg硫酸锌)、氟加中锌组(25mg/kg氟化钠+20mg/kg硫酸锌)、氟加高锌组(25mg/kg氟化钠+50mg/kg硫酸锌),每组10只,灌胃6周。观察氟、锌对精子数量、活动率和畸形率、血清性激素、睾丸生化标志酶及睾丸组织病理学切片的影响。结果:①精子计数、活动率:氟加中锌组大鼠精子计数、精子活动率均高于氟组、氟加低锌组和氟加高锌组,差异均有统计学意义(P<0.05);精子畸形率:氟加中锌组大鼠精子畸形率低于氟组、氟加低锌组和氟加高锌组,差异均有统计学意义(P<0.05)。②血清睾酮含量:氟加中锌组血清睾酮含量高于氟组和氟加高锌组,差异有统计学意义(P<0.05)。③睾丸乳酸脱氢酶活力:氟加中锌组高于氟加高锌组,差异有统计学意义(P<0.05)。睾丸氟含量:氟加中锌组低于氟组,差异有统计学意义(P<0.05)。④睾丸组织病理学检查结果:氟组、氟加高锌组与对照组比较,形态结构疏松;氟加低锌组、氟加中锌组与氟组比较,结构较完整。结论:中等剂量的锌可以拮抗高氟所致的雄性生殖毒性。更多还原

【Abstract】 Aim:To explore the antagonism of different doses of zinc on male reproductive toxicity induced by excessive fluorine.Methods:A total of 50 male Wistar rats were randomly allocated into five groups:control group,fluorine group,fluorine plus low-dose zinc group,fluorine plus mid-dose zinc group,and fluorine plus high-dose zinc group. The animals were fed with deionized water supplemented with NaF or NaF and ZnSO₄ for 6 weeks.The count of sperm and the rates of sperm mobility and aberration were analyzed.The levels of lactate dehydrogenase（LDH） and serum testosterone（T） were examined.Meanwhile the pathological alterations of testes were observed.Results: Compared with fluorine group, fluorine plus low-dose zinc and fluorine plus high-dose zinc group,the counts of sperm and the rate of sperm mobility significantly increased, and the rates of sperm aberration significantly decreased in fluorine plus mid-dose zinc group （P<0.05）. The levels of T in fluorine plus mid-dose zinc group were significantly increased（P<0.05） compared with fluorine group and fluorine plus high-dose zinc group （P<0.05）.The levels of LDH were significantly higher in fluorine plus mid-dose zinc group than that in fluorine plus high-dose zinc group. The content of fluoride in fluorine plus mid-dose zinc group was significantly lower than that of fluorine group （P<0.05）. The microstructure of testis was loosened in fluorine group and fluorine plus high-dose zinc group compared with control group,while the pathological alterations were milder in the fluorine plus low-,mid-dose zinc groups compared with fluorine group.Conclusion: Fluoride can result in reproductive toxicity in male rats,and appropriate zinc can antagonize the toxicity of fluoride.更多还原