【摘要】 分别采用基础平板培养法和富集培养法,从河南烟叶表面分离筛选尼古丁降解菌。结果表明:采用基础平板培养法分离出来的30个菌株中,有效菌株仅占所分离菌株的60.0%,对尼古丁降解效率大于30%的仅有6株菌(37℃,24 h)。而富集培养法分离出来的30个菌株中,有效菌株占所分离菌株的比例达93.3%,对尼古丁降解效率大于30%的有14株菌,表明富集培养法是一种快速高效分离筛选尼古丁降解菌的理想方法。经初步鉴定,分离到的2株具有较高活性的尼古丁降解菌分别为短芽孢杆菌(B.brevis)和侧孢芽孢杆菌(B.laterosporus)。更多还原

【Abstract】 Nicotine-degrading microbes were Isolated from Henan tobacco leaves by basic culture and enrichment culture methods.The results showed that the effect of enrichment culture is better than that of basic culture in isolation and selection of nicotine degrading microbes.The ratio of valid strains isolated by basic culture method was 60.0%,while that by enrichment culture method was 93.3%.The stain number with nicotine degradation rate of more than 30% was 6 out of the 30 strains isolated from the basic culture,and 14 from the enrichment culture.Based on th physiology and biochemistry characters,2 strains with higher nicotine-degrading activity were identified,i.e.B.brevis and B.laterosporus.更多还原