【摘要】 通过对粳稻品种日本晴及5个籼稻品种的研究,发现籼稻、粳稻在成熟胚愈伤组织培养、农杆菌遗传转化的最适条件方面存在差异.在MS,CC,NB培养基上,日本晴的愈伤组织诱导率达86.37%以上,远高于各籼稻品种;与农杆菌液的共培养时间,籼、粳稻均以3 d为宜;转化后的籼稻抗性愈伤组织用40 mg/L的潮霉素筛选效果较好,日本晴则需用50 mg/L的潮霉素筛选;幼苗生根阶段,籼稻的潮霉素适宜质量浓度为20～30 mg/L,日本晴的潮霉素适宜质量浓度为50 mg/L;日本晴的gus基因瞬时表达率强于各籼稻品种,hpt基因转化率也高于各籼稻品种.更多还原

【Abstract】 Different response to A.tumefaciens-mediated transformation of Nipponbare(japonica rice) and five indica rice isolates were investigated.The results are as follows:the inducement rate of Nipponbare reached 86.37%,on MS,CC,NB medium significantly higher than that of the indica rice isolates examined.For both indica and japonica variety,co-culture with tumefaciens for 3 days was beneficial to the target gene’s transformation;40 mg/L hygromycin could be used for selection of indica rice transformants selection,but 50 mg/L was needed for Nipponbare;20～30 mg/L hygromycin is beneficial for selecting positive transplants of incica rice,and 50 mg/L for Nipponbare;Gus gene transient expression of Nipponbare and hpt gene transformation efficiency were better than that of indica isolates.更多还原