【摘要】 为了探讨利用死亡动物皮肤组织获取成纤维细胞的方法,研究将小鼠胎体或皮肤分别在4℃的mPBS液中保存24~120 h,然后通过原代培养、冷冻复苏和继代培养,观察了皮肤成纤维细胞的生长增殖能力。结果表明:与新鲜皮肤(对照组)原代培养成纤维细胞达到85%以上汇合需要8 d相比,4℃保存24~120 h后的皮肤(试验组)需要的时间延长(9~21 d),但仍能贴壁生长并达到85%以上汇合,且皮肤保存法优于胎体保存法;各试验组经3~4 d继二代培养即可达到85%汇合(对照组为3 d);冷冻复苏的各试验组原代培养细胞,经继二代培养即可在3~4 d内达到85%以上汇合。说明动物死亡后,若及时采集其皮肤并在4℃的mPBS液中保存一段时间,经原代培养和继代培养,或经原代培养、冷冻复苏和继代培养,可获得具有正常继代能力的成纤维细胞。更多还原

【Abstract】 To explore the utilization of somatic cells of dying mammals,this study observed the proliferating capability of skin fibroblasts by primary culturing,frozen-thawing,and subculturing of fetus mice skin stored at 4 ℃ for 24～96 h.The results showed that: comparing to the fibroblasts from fresh skin tissues,which needed 8 days to get to more than 85% confluence,the ones from mouse fetus stored at 4 ℃ required 9 to 21 days to get to more than 85% confluence.Moreover,storing skin only was better than storing the whole fetus.The fibroblasts from different experimental groups grew to more than 85% confluence in 3~4 days at the second subculturing at the same time.After frozenthawing,the primarily cultured fibroblasts from different experimental groups could confluenced to 85% in 3~4 days.These results indicated that once the animals died,their skin were collected and stored at 4 ℃ in mPBS for a short time, the fibroblasts could be got from the stored skin through primary culturing,frozen-thawing,and subculturing.更多还原